Worked example to determine optimum cell density and incubation time for a549 cells, using alamarBlue® and spectophotometry
The cell line a549 is a monolayer culture. The method given was followed to find the percentage reduction of alamarBlue® after incubation with a549 cells at different densities and for different incubation periods.
Absorbance values at 570 nm and 600 nm after blanking with media only for a549 cells.
Using absorbance data from the sample data set, percent reduction of alamarBlue® was calculated using equation 1:
Percentage reduction of alamarBlue® after incubation with a549 cells at different cell densities and incubation periods.
These values are plotted to produce Figures 1a & 1b.
Graphs showing percentage of alamarBlue® reduction for a549 cells with different incubation periods (Figure 1a) and at different initial cell densities (Figure 1b).
Click here for notes to accompany these graphs.
